Heat-Shock-Induced Proteins from Myxococcus xanthus

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Heat-shock-induced proteins from Myxococcus xanthus.

Optimal conditions for two-dimensional gel electrophoresis of total cellular proteins from Myxococcus xanthus were established. Using these conditions, we analyzed protein patterns of heat-shocked M. xanthus cells. Eighteen major spots and 15 minor spots were found to be induced by heat shock. From N-terminal sequences of 15 major spots, DnaK, GroEL, GroES, alkyl hydroperoxide reductase, aldehy...

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Characterization of a small heat shock protein, Mx Hsp16.6, of Myxococcus xanthus.

A number of heat shock proteins in Myxococcus xanthus were previously identified by two-dimensional (2D) gel electrophoresis. One of these protein was termed Mx Hsp16.6, and the gene encoding Mx Hsp16.6 was isolated. Mx Hsp16.6 consists of 147 amino acid residues and has an estimated molecular weight of 16,642, in accordance with the apparent molecular mass in the 2D gel. An alpha-crystallin do...

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Identification of enhancer binding proteins important for Myxococcus xanthus development.

Enhancer binding proteins (EBPs) control the temporal expression of fruiting body development-associated genes in Myxococcus xanthus. Eleven previously uncharacterized EBP genes were inactivated. Six EBP gene mutations produced minor but reproducible defects in fruiting body development. One EBP gene mutation that affected A-motility produced strong developmental defects.

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 2001

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.183.21.6282-6287.2001